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walR [2019-04-18 09:28:41]
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walR [2019-04-18 09:28:41]

two-component response regulator ([SW|OmpR family]), controls cell wall metabolism
locus
BSU40410
pI
4.88
mw
27.08 kDa
protein length
235 aa Sequence Blast
gene length
708 bp Sequence Blast
function
control of cell wall metabolism
product
two-component response regulator ([SW|OmpR family])
essential
yes
synonyms
yycF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

    • Gene

    Coordinates
    4,153,696 4,154,403

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • essentiality can be bypassed by constitutive expression of [gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] or [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] in a [gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC] [gene|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA] mutant background [pubmed|29465029]
  • a constitutively active form of [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR] ([protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]-R204C) results in constitutive expression of the [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR] regulon, and in combination with a ''[gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF]'' mutation to the formation of cell wall-less L-forms [Pubmed|22122227]

    • The protein

    Protein family

  • [SW|OmpR family] of two-component response regulators

    • Paralogous protein(s)

  • [protein|6A37531896205A9B894C81AB0563C216C0B52CD7|YkoG], [protein|706983E6942E883D3A9D45693E7B4015AEABE60B|YclJ], [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]

    • Modification

  • phosphorylated by [protein|E88978809272FAC2520DB4ABCA0554F8028F3451|WalK] on an Asp residue
  • phosphorylated by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC] on Thr-101, this stimulates [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR] activity [Pubmed|26102633]

    • Effectors of protein activity

  • phosphorylation likely affects DNA-binding activity

    • Structure

  • [PDB|2OQR] (from Mycobacterium tuberculosis, 48% identity) [pubmed|17942407]
  • [PDB|2D1V] (DNA binding domain)[PDB|2ZWM] (receiver domain)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • additional information

  • information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000217 PRODORIC2 database]

    • Expression and Regulation

    Operons

    genes
    [gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]-[gene|E88978809272FAC2520DB4ABCA0554F8028F3451|walK]-[gene|06D2D03C65D50E3909B2C93835193815B6016967|walH]-[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]-[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]-[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]
    description
    [Pubmed|9829949]

    regulation

  • ''htrC'' transcript expressed during sporulation [Pubmed|9829949]
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    Biological materials

    Mutant

  • MGNA-B828 (yycF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1827 NBRP B. subtilis, Japan]

    • Expression vectors

  • pGP2633, constitutive expression in ''B. subtilis'' (based on [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP2635, expression of His-[protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR] in ''E. coli'' (based on [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • labs

  • [SW|Kevin Devine], Dublin

    • References

    Reviews

  • 19019149,18245295,18792692,22126136,10094672,28886686

    • The [SW|AbrB regulon]

  • 20817675,18840696,23580539

    • The [SW|WalR regulon]

  • 17581128,10878122,12950927,23199363

    • Other original Publications

  • 18573169,17350627,10094672,17827301,9829949,16030236,20111065,20167622,20834167,24125693,22122227,24125693,24490131,18789936,26102633,17942407